Sections were in that case washed with 2 M NaCl/50 mM EDTA/1x protease inhibitors/20 mM Tris, pH 7
Sections were in that case washed with 2 M NaCl/50 mM EDTA/1x protease inhibitors/20 mM Tris, pH 7.4 for 10 min at 4C followed by 100 mM NaCl/20 mM Tris for 1 h at 4C. long range scanner. The above images are amplitude images with a scan size of 5…