Na/K ATPase 1 subunit and actin (loading settings) were detected having a mouse anti-Na/K ATPase 1 subunit antibody (Millipore, 05C369; 1/10,000), and mouse or rabbit anti-actin antibody (Sigma, A2228 and A2066; 1/1,000 for both), respectively

Na/K ATPase 1 subunit and actin (loading settings) were detected having a mouse anti-Na/K ATPase 1 subunit antibody (Millipore, 05C369; 1/10,000), and mouse or rabbit anti-actin antibody (Sigma, A2228 and A2066; 1/1,000 for both), respectively. Macropinocytosis live imaging Twenty-four hours before imaging, 1.5??105 BMDMs were seeded in M-CSF-free complete DMEM…