Supplementary MaterialsAdditional file 1

Supplementary MaterialsAdditional file 1. compounds for various cell selective imaging probe development, which was named Diversity Orientated Fluorescence Library Approach (DOFLA) [14]. A high-throughput system using the in-house DOFLA [15C17] was employed to screen 46 fluorescent probes that we predicted may specifically recognize pluripotent stem cells. Twenty-nine out of the…

Use the BM Chemiluminescence Western Blotting kit (Roche) to detect the immunoreactive bands

Use the BM Chemiluminescence Western Blotting kit (Roche) to detect the immunoreactive bands. Cell Proliferation Assay Cell viability and proliferation were assessed using CCK-8 kit (Beyotime Biotechnology). concentrations and hyperpolarization increased after over-activation of AMPK using the specific activator AICAR or suppression of CFTR activity using specific inhibitor GlyH-101. Under…