Beta-actin was detected as the endogenous control for (ISOC1 OE) and miR-4633 stably overexpressed NSCLC cell lines, the full-length of and miR-4633 were amplified from complementary DNA (cDNA) and genomic DNA (gDNA) from the NSCLC cell line A549 by PCR and were cloned into the pCDH-CMV-MCS-EF1-GFP+Puro (CD513B) plasmid (System Biosciences, USA)

Beta-actin was detected as the endogenous control for (ISOC1 OE) and miR-4633 stably overexpressed NSCLC cell lines, the full-length of and miR-4633 were amplified from complementary DNA (cDNA) and genomic DNA (gDNA) from the NSCLC cell line A549 by PCR and were cloned into the pCDH-CMV-MCS-EF1-GFP+Puro (CD513B) plasmid (System Biosciences,…

Ciejek E

Ciejek E. The mating phenotypes of the candida populations were clearly observed over hours. Three distinct reactions were observed depending on the distance between the A- and -cell populations: the cells either continued to divide, arrest, and develop a stereotypical polarized projection termed a shmoo toward the cells of reverse…