Charcoal-bound radioactivity was assessed on the Beckman LS 6500 scintillation counter-top
Charcoal-bound radioactivity was assessed on the Beckman LS 6500 scintillation counter-top. DdaC/D Enzymatic Assays with Anaerobically Purified DdaC Phosphopantetheinylation reactions (12C270 L) contained 25 M DdaD, 12 M Sfp, 400 M coenzyme A (CoA), 10 mM MgCl2, 1.5 mM dithiothreitol (DTT), and 50 mM HEPES (pH 7.5). Services from the…