Recently, Xie et al.11, revealed the detection of a total of 88 common DE miRNAs and 120 DE novel piRNAs in both serum and urine samples from infected rabbits. AUC 0.859 indicates good discriminatory power. Examined synthetic drugs and natural products can target specific amino acids residues of TgERP that lie at the same binding Squalamine interface with LB1 and Ki67, subsequently, hindering their conversation. Hence, salivary samples can be a promising diagnostic approach. The studied drugs can counteract the pro-inflammatory action of TgERP. Keywords:Salivary, Sera, Discrimination, Docking, Drug, Data, Bank Subject terms:Computational biology and bioinformatics, Drug discovery, Immunology, Microbiology, Biomarkers, Medical research, Risk factors == Introduction == Toxoplasma gondiiis a cosmopolitan zoonotic protozoan parasite1. The global prevalence of latent toxoplasmosis in pregnant women was estimated to be 33.8%2. The seropositivity for anti-ToxoplasmaIgG among diabetic patients from Cairo, Egypt was 46%. A higher rate was revealed among pregnant women (67.5%) in Menoufia3,4. Humans can be infected by ingesting tissue cysts in uncooked meat or from an infected mother to her fetus. The latter may result in stillbirth, abortion, and congenital abnormalities including chorioretinitis, microcephalus, intracerebral calcifications, and hydrocephalus5,6. In addition, contamination can be acquired by ingesting oocysts in unsanitary environments via contaminated food and water7. While it is usually asymptomatic and self-limited in immunocompetent individuals, it can cause ocular or neurological disorders in immunocompromised patients8. It can be accurately diagnosed by various methods, including serological assessments, polymerase chain reactions, histological examination, and organism isolation9. Traditionally, blood samples have been the primary source of serological testing. However, researchers have been exploring option sample sources to improve testing convenience and patient compliance10. Body fluids, especially blood, saliva, and urine, are rich resources for potential diagnostic biomarkers for a number of Squalamine diseases11. Mahmoud12demonstrated that urine samples could be easily and readily collected without causing any inconvenience to the study participants. The samples showed high sensitivity toT. gondiiantigens by reverse latex agglutination test and enzyme-linked immunosorbent assay (ELISA) among patients with acute toxoplasmosis. Additionally, several studies have focused on investigating toxoplasmosis in animals. For instance, Huskinson et al.13detectedToxoplasmaantigens in urine of acutely infected mice as well as patients with acute toxoplasmic encephalitis. Years later, Hu et al.14, detected theToxoplasmaB1 gene in urine samples of infected mice using the LAMP method. Recently, Xie et al.11, revealed the detection of a total of 88 common DE miRNAs and 120 DE novel piRNAs in both serum and urine samples from infected rabbits. Saliva has gained attention as a non-invasive and readily accessible biofluid that may offer a reliable option forT. gondiiserological testing15. Assessments using oral fluid have been developed for viral infections including HIV16, hepatitis17, norovirus18, and cytomegalovirus19, as well as bacterial infections such asHelicobacter pylori18, and parasitic infections likeT. gondiiandCryptosporidium parvum18,20. Oral fluid comprises crevicular fluid that is notably rich in IgA, IgG, and IgM21. Most IgG in saliva is derived MCM7 from serum and enters the oral cavity via crevicular fluid whereas most IgA in oral fluid is usually produced in the salivary glands, reflecting both mucosal and systemic immunity22. IgM is present at lower concentrations in salivary fluid than IgG and IgA23. The additional complexity of toxoplasmosis is usually attributed to its tightly regulated life cycle, which alternates between sexual developmental stages in felines and multiple asexual forms in a wide range of intermediate hosts. The Squalamine host immune response stimulates the tachyzoites to switch back to bradyzoites that encyst in skeletal muscle and brain tissues to establish a chronic contamination. All this paid attention to exploring high-efficacy therapy targetingToxoplasmaproteins-induced immunological disorders based on serological assessments24. Epidemiological studies that could lead to the development of strategies to reduce infection in humans and food animals are made difficult by the lack of assessments that can distinguish between the contamination routes of ingestion of tissue cysts in undercooked meat and oocysts in cats feces25. Among the numerous immunogenic proteins inT. gondiilate embryogenesis abundant domain-containing Squalamine proteins are crucial for pathogen survival and resistance to high salinity, drought, and freezing conditions. Of these, embryogenesis-related protein ERP serves as a sporozoite-specific antigen T. gondiiembryogenesis-related protein (TgERP) and corresponds to the TGME49_076850 gene25,26. Squalamine IgG antibodies against TgERP are more prevalent than non-specific IgG and aid in discriminating between oocyst and bradyzoite infections27. While TgERP helpsT. gondiisporozoites to persist for long periods in harsh environmental reservoirs without a host, its short-lasting nature (about 68 months) and the relatively short half-life of anti-TgERP antibodies limit its applications in epidemiological assessment studies26,28. The presence of this antibody differentiates contamination in.