The general objective of this study was to assess whether dynamic temporal, structural, and positional changes of nucleosomes influence the sepsis phenotype
The general objective of this study was to assess whether dynamic temporal, structural, and positional changes of nucleosomes influence the sepsis phenotype. THP-1 sepsis cell model to isolate mononucleosomes by quick cell permeabilization and digestion of chromatin with micrococcal nuclease and then compared tumor necrosis element (TNF) proximal promoter nucleosome…