Description of terms or pathways with same color indicates similar function
Description of terms or pathways with same color indicates similar function. 12983_2020_366_MOESM5_ESM.pdf (248K) GUID:?DE78AF05-FA8E-4D84-B741-5A6DDE94E384 Additional file 6: Table S1. analysis on genes in TP-M9, TP-M10 and AS-M1 modules identified in WGCNA. Description Pentostatin of terms or pathways with same color indicates similar function. 12983_2020_366_MOESM5_ESM.pdf (248K) GUID:?DE78AF05-FA8E-4D84-B741-5A6DDE94E384 Additional file 6: Table S1. Sequencing data collected from RNA-seq. Table S2. Summaries of assemblies generated by TRINITY. 12983_2020_366_MOESM6_ESM.xlsx (15K) GUID:?0B81CD89-B11F-452D-B53B-DC764B319573 Additional file 7: Table S3. Details of DEGs identified in each pairwise comparison of four physiological states using edgeR. 12983_2020_366_MOESM7_ESM.xlsx (292K) GUID:?EFD4DE20-2E56-45ED-A751-AED763CA515D Additional file 8: Table S4. Details of DEGs identified in each pairwise comparison of four physiological states using DESeq2. 12983_2020_366_MOESM8_ESM.xlsx (233K) GUID:?76404449-57FD-47F8-9E7D-1A5DE3B6821A Additional file 9: Table S5. Results of WGCNA module detection of 1614 DEGs and mean log2CPM of DEGs in each of the four states. Table S6. Gene ontology (GO) terms enriched in DEGs identified in each pairwise comparison of states. Pentostatin Table S7. KEGG and Reactome pathways enriched in DEGs identified in each pairwise comparison of states. Table S8. Summary of KEGG and Reactome pathways and GO terms enriched in DEGs in each WGCNA module. Table S9. Details of DEGs included in PPI network of module TP-M9 associated with Torpor. Table S10. Details of DEGs included in PPI network of TP-M10 module associated with Torpor. Table S11. Details of DEGs included in PPI network Pentostatin of module AS-M1 associated with Arousal. Table S12. DEGs upregulated in winter torpor relative to summer active that were shared by the three tissues (intestine, liver and brain) in and and and several DEGs (e.g. and [22, 23]. Additionally, a recent study on this species has indicated that physiological changes during hibernation might alter the gut microbiota community [32]. However, little is Pentostatin known about the response of the gut to hibernation in this species. Here we use comparative transcriptomics to investigate functional changes of the gut during hibernation. In particular, we focus on two primary gut functions: food intake regulation and immunity. By examining the expression differences of thousands of genes among bats from winter torpid, arousal and summer active states, we test three hypotheses: Rabbit Polyclonal to KAPCB 1) In response to the stress of luminal nutrient deficiency during hibernation, genes and/or gene networks related to regulation of food intake would be upregulated in winter torpor relative to summer active; 2) In order to protect the gut from microbial and inflammatory damage, genes involved in innate immune system will be upregulated in winter torpor and arousal of bats; 3) In order to recover from the torpid state quickly, genes associated with cell proliferation (e.g. cell cycle and cell division) would be overexpressed in winter active bats relative to torpid Pentostatin bats. Moreover, we compared our current results from the gut with previous findings in the brain and liver of to investigate molecular effects of torpor on different tissues of hibernating animals. We predicted that differentially expressed genes involved in coping with hypothermia stress would be shared by all of the three tissues. Methods Experimental design and sampling In this study we captured 15 adult male individuals of the greater horseshoe bat (individuals based on expression data of 14,009 genes. a Four physiological states included in this study (Torpor, Arousal, Winter Active, and Summer Active) with their corresponding rectal temperatures; b Principal component analysis (PCA) showing gene expression distance of all individuals; c Hierarchical clustering analysis based on pairwise Pearson correlation of gene expression in all individuals For tissue sampling, bats were rapidly sacrificed by cervical dislocation. Then the small intestine near the stomach was taken and transferred to RNase-free PCR tubes. To reduce the amount of gut.